Topic outline

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  General

  • News forum
  • Discussion about topics of the Course Forum

    Students can use this forum to discuss topics of the course - ask questions - answer collegue's questions - suggests web links or scientific articles relevant for the course

  
  

  Topics index:
  

  • Introduction
  • Conferences _ Events
  • Experimental approaches/methods and animal models
  • FOCUS GROUP
  • Student presentations
  • Basic concepts in Developmental Biology
  • Neural Induction
  • Neural Patterning
  • Neuro-/Glio-genesis
  • Development of neurons controlling reproduction
  • Cerebral Cortex development
  • Neuronal development
  • Adult neurogenesis
  • Exams

  
  
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  Introduction

  • Introduction File

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  • Video introduction - Developmental Neurobiology (JOVE) File
  • Useful links

  • UNSW - embryology - neural system development URL

    Embryology online education and research website of the University of New South Wales, Sydney, Australia.
    

  • 3D nervous system development - (mouse) URL
  • Allen Brain Atlas URL
  • Developmental Biology (textbook 6th edition online)yy URL
  • NEW!!!!! 3D transparent Human embryo Atlast URL

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  Conferences _ Events

  • NEW!!!! Student's Presentation - @DBIOS aula B (9-12am) May 12 File

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  • NEW!!! Genetics of Brain Disorders@MBC 29 May 2017 File

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  • NEW!!!! Workshop Microscopia - 30 May 2017 File

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    14.00 Sara Bovetti (IIT)

    High-speed in vivo two-photon calcium imaging of

    cortical and subcortical neuronal networks

    14.40 Stefano Fumagalli (Mario Negri)

    Structured illumination microscopy as an approach to

    study phagocytosis in the ischemic brain

    15.20 Guido Serini (IRCCS - Candiolo)

    Dissecting the underpinnings of vascular cell adhesion

    dynamics

  • Pint of Science URL

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  • Pint of Science - 2017 File

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  • Lecture by Prof. Yoav Gothilf File

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  • ppt presentation Gothilf File

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  • Conferences: Settimana del Cervello URL

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  • Brain awareness week_Dana Foundation URL
  • Summer school in Neural circuit development and Brain plasticity URL

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    The summerschool is based in Utrecht and is aimed at advanced master students and starting PhD students with the goal to teach them on recent advances in neural circuit development and plasticity from an interdisciplinary perspective. 

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  Experimental approaches/methods and animal models

  • Video_in utero electroporation in mice URL
  • In ovo electroporation of chicken embryos URL

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  • ANIMAL MODELS

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  • An Introduction to the laboratory mouse URL

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  • Mouse reproduction and development URL

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  • Mouse care/maintenance URL

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  • Drosophila melanogaster URL

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  • Drosophila development and reproduction URL

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  • Introduction to C. elegans as a model system_video URL
  • C. elegans reproduction and development URL

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  • NEW!!! Introduction to zebrafish URL

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  • NEW!!! laboratory of development and disease models in zebrafish URL

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  • ORGANOIDS

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  • Orchestrating organoids - The Scientist URL

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  • CED conference by M.Lancaster URL

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  • Lancaster et al., Nature 2013 File

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  • Large brains appear several times in the mammalian radiation: have a look to the picture! File

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  • IMAGING

    
    

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  • Microscopy: choosing the right microscope_video URL

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  • Confocal microscopy basics-video URL

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  • Two-photon microscopy_basic URL

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  • Light-sheet microscopy webinar URL

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  • Light-sheet microscopy File

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  • 3D-human embryo development - Belle et al., Cell 2017 File

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  • Cellpapere-clip interview to Alain Chedotal on transparent human embryo URL

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  • live imaging of mitosis in the developing mouse embryonic cortex _method URL

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  • Time lapse protocol _ Noctor 2011 File

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  FOCUS GROUP

  This activity will be scheduled for each wednesday (unless otherwise specified) - Students are invited to participate and to prepare by performing the task assigned the week before, in order to be able to be an active participant in the class.

  • Focus group 1 - march 15 -Study of embryogenesis in vitro

    Task: reading the article by Harrison et al., 2017 Science

    
    

    Focus group 2 - march 22 - Brain organoides

    Task: reading the article by Lancaster et al., 2013 Nature

    --> a message has been sent through the forum about today's discussion on microcephaly

    
    

    Focus group 3 - march 29 - Neural induction

    Task: reading the article by Marchal et al., 2009 PNAS

    
    

    Focus group 4 - april 5 - Neural patterning

    Task: reading the article by Lippmann et al., 2015 Stem cell reports

    
    

    Focus group 5 - may 17 - Cortical development

    Task: reading the article by Harb et al., eLife 2016

    
    

    
    

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  • discussion focus group 1 Page

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  • discussion focus group 2 Page

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  • discussion focus group 3 Page

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  • discussion focus group 4 Page

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  Student presentations

  
  

  - read the editorial overview: Developmental neuroscience 2017 (Current Opinion in Neurobiology)

  - select one of the reviews of your choice among those cited in the editorial 

  - prepare a presentation to be given in the class

  for this task students can work alone or in small group  

  • Editorial overview: Developmental neuroscience 2017 File

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  • NEW!!! students presentation Assignment

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    please upload here your presentation in a pdf format  (max size for each file 64MB)

  • Beyond proneural - by Canicatti AND Nicola File

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  • Adult neurogenesis and brain plasticity by Bozkurt, Mulè and Rossi File

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  • Neural circuits tuning and interneurons by Michelon and Zuccarini File

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  • Interplay between neurons and glia at the synapse by Ghibaudi and Stajano File

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  • Feedback on students presentations File

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  Basic concepts in Developmental Biology

  Starting from the fact that a new complex organism originates from a single totipotent stem cell (the zygote) through development, and tissue specific differentiated cells can arise from single pluripotent stem cells, you will be guided to reflect on the very basic cellular mechanisms underlying such processes. You will read about cell differentiation, specification, determination, fate and commitment ...
  

  • Basic concepts - with audio commentary File

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  • Key-Notes: basic concepts in Developmental Biology Book

    Key-Notes

  • TASK - reading articles 1.

    1. read the article by Harrison et al., 2017 following suggestions you will find in the file notes on scientific articles;

    2. take notes that will be useful to discuss the article during the lesson on Wednesday - March 14;

    3. identify terms used in the article that are new for you and create a glossary (max 10 terms each student)

    
    

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  • Notes on scientific articles File

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  • Harrison et al., Science 2017 File

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    In this article published on Science (March 2, 2017) researchers describe how, using a combination of genetically-modified mouse ESCs and TSCs, together with a 3D scaffold known as an extracellular matrix, they were able to grow a structure capable of assembling itself and whose development and architecture very closely resembled the natural embryo
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  Neural Induction

  Main objective of this unit is the understanding of early neural development 
  

  We will discuss about:

  -derivation of neural tissue in different metazoans

  -the origin role and molecular nature of the neural inducer

  -conservation of the neural inducer among metazoans

  
  

  
  

  
  

  • Derivation of the neural tissue File

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  • Notch signalling pathway URL
  • Neural induction File

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  • Key-notes on Neural Induction Book

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  • Video_Amphibian development_gastrulation URL

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  • Video_Gastrulation_neurulation (Xenopus laevis) URL
  • Video_Spemann and Mangold experiment URL
  • BMP signaling pathway URL
  • Marchal et al., PNAS 2009 File
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  Neural Patterning

  This unit is focussed on the  understanding of the mechanisms that underly the regional identity of the nervous system.

  We will discuss about:

  - antero-posterior axis whit a focus on Hox genes function in hindbrain development; 

  - organizing centers in the developing brain;
  

  - dorsal-ventral polarity of the neural tube.

  • neural patterning_1 File

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  • Wnt / β-Catenin Signaling Pathway URL
  • Lippmann et al., 2015 File

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  • Neural patterning_2 File

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  • Hedgehog Signaling Pathway URL
  • Watch the lecture by Tom Jessell

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  • Building Brains: The Molecular Logic of Neural Circuits URL
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  Neuro-/Glio-genesis

  • Astrogliogenesis in the cerebellum File

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  • Masquarque et al., 2012 File

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  • Grimaldi et al., 2009 File

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  • Radial glia File

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  • Time-lapse video - RG URL

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  • Noctor et al., 2004 File

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  Development of neurons controlling reproduction

  • lecture File

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  • video OB File

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  • Messina et al., 2016 File

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  • Casoni et al., 2016 File

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  • video 1 File

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  • video 2 File

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  Cerebral Cortex development

  • Cerebral cortex development_1 File

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  • Cerebral cortex development_2 File

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  • cerebral cortex neurogenesis URL
  • Harb et al., 2016 File

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  • The Developing Cortical Neuron Transcriptome Resource - web site URL
  • NEW - Sizing up the brain gene by gene - by Christopher Walsh URL

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  Neuronal development

  • Axon growth and regrowth File

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  • How axons grow URL

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  • Rho-GTPases in neuronal development File

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  • article Zamboni et al., Sci Rep 2016 File

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  Adult neurogenesis

  • Adult neurogenesis File

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  • presentation on COUP-TFI and DG neurogenesis URL

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  • Watch the video by Fred Gage!!!

  • Fred Gage talks about adult neural stem cells URL
  • Brain repair and regeneration File

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  Exams

  • 22_06_2017 Quiz

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  • exam 13_07_2017 Quiz

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  • 20_09_2017 Quiz

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