Article by Lippmann et al., 2015

• Explain the following sentence: “during formation of the posterior CNS, progenitors proximal to the node progressively transition from 3’ to 5’ Hox expression profile as the primitive streak regresses”.
  
• What is the rationale of the study?
  
• Why they choose to work with monolayer cultures instead of 3D approaches (e.g. organoids)
  
• What were the general protocols for in vitro Hox patterning in hPSC before this study? What were the main limits?
  
• In which way the authors were able to optimize the in vitro protocols to stabilize neuromesodermal progenitors?
  
• What is the limit in the analysis of transcript levels?
  
• Try to simplify the main message of this study in a schematic drawing with title+legend
  

Students: Daniele Stajano, Marco Ghibaudi, Viviana Gatti, Mulè Francesca, Zuccarini, DiRenzo, Rossi, Micheloni, Celeste Nicola