05-Exercise II
Opened: Monday, 22 November 2021, 9:00 AM
I Exercise:
• identify the buffers to digest with
enzyme 1 and enzyme 2 the vector with the insert (pCR-bluntII-TOPO) and the vector for the following
subcloning (pEGFP-C3)
As we do in the in notebook in the lab, write the protocol for all necessary passages to digest the vector with the insert (pCR-bluntII-TOPO) and the vector for the following subcloning (pEGFP-C3):
• first digestion (write all ingredients
of the first reaction)
• purification of the DNA?
• second digestion (write all ingredients
of the second reaction)
OR
- first & second digestion together (write all ingredients of the reaction)