Exercize: prepare a single slide like the one shown during my lecture, containing all necessary information:

1-the entire sequence from EGFP to the antisense primer, highlighting the primers, the restriction sites, the ATG, the STOP codon)

2- the vector used (1, 2 or 3)

3- restriction sites used (do not use HindIII!!)

4- the primers (written correctly, with restriction sites in bold)

5- the protein translation

6- the full map showing the fusion protein