Copy and past the following questions and use telegraphic style (omitting articles, pronouns, conjunctions, transitions, and other sentence structures) in your answers.

What did you learn from the remote readings and materials? (indicate, according to your personal background, which information from the remote materials (including lectures from G. Gambarotta) is the most useful to you and your take home messages. There is no right or wrong answer. 

......

What did you learn from paper 03? (This will help you participating to paper discussion during the lecture).

Answer the following questions:

1. Which aspect, if any, is the most:
1. interesting to you? .....
2. difficult to you ....  
2. What additional experiments would you perform to follow up on this work? .......

Note: Evaluation (excellent to poor) is according to commitment and response elaboration, not a matter of length of the response. Stay focused.

Specific questions:

1. What was the motivation for conducting this study? 
2. Which is the main role of SH2 domains in signal transduction? Explain why  in CBR-ABL tyrosine kinase, SH2 domain is a potential target to inhibit CBR-ABL tyrosine kinase activity?
3. Which technique would you use to introduce I164E and T231R single mutations and the I164E/T231R double mutation into Bcr-Abl ? (give only the name of the technique)
4. Figure 1B: Which results allow the author to conclude that CBR-ABL I164E mutation is dominant with respect to T231 mutation ? (indicate: figure left or right; blot number from top to bottom and lane number from left to right).
5. Figure 2C, blots 2-4: Explain the role of the last 3 blots and the reason for preparing only 3 blots while transfecting 4 constructs?
6. Figure 2 C, blot 1: -pY evidenced bands with molecular weight about 70 and 50KD of MW, corresponding respectively to proteins …..
7. In figure 4 and in the text (p311), authors observed and discussed the fact that  CBR-ABL I164E confers growth factor independence to Ba/F3 and UT-7 cells despite reduced kinase activity. What is the general take home message with respect to results obtained with transfection/transduction experiments?
8. Figure 5: Which is the main conclusion of figure 5 experiments?
9. Figure 6: Which homologies can you figure out between the immune system (which is prepared to respond to unknown antigens) and a technical approach such as phage display? 
10. Explain similarity and difference between fig 3 D and fig 7A and 7B