Anyway, if the professor will read our questions later mine are: 1) When transposons are translated they can recognize only the TIRs of their membership sequences or they can also relocate other sequences that contain TIRs 2) If only the promoter is transponed (?, is the correct terminology?), is it possible to have the unproperly translation of oncogene (tumor development)? So, we can consider trasposome like a tumor cause?

good morning everyone

Hi Allan

i would like to ask if what exactly anotation means in genomes

I'm not understanding

tell me what you need now

Okay there are several question

yes I've seen past questions

Great

Goddmorning prof

i was wondering about anotation because the dictionary meaning is adding a comment to something

if one says that genes are anotated, does it mean that they are commented

sorry for the redundant question

beyond those questions we were wondering why Activity 2 was reopened, do we have to do it again?

I still can see my previous attempt

i am engaged in a phone call at the moment, could you wait 5 min please

no was for people that could'nt access before

OK i'm back

concernign annotation: of course when scientists have a sequence of a fragment of DNA thay try to understand what it is

e.g thay find similarity with a retrovirus, they call it "LTR"

this is annotation

unfortunately Allan has left

OK any other question?

I'll copy and paste Cecilia's questions if you want

) When transposons are translated they can recognize only the TIRs of their membership sequences or they can also relocate other sequences that contain TIRs 2) If only the promoter is transponed (?, is the correct terminology?), is it possible to have the unproperly translation of oncogene (tumor development)? So, we can consider trasposome like a tumor cause?

transposed

Ye your guess is correct: the jumping of DNA transposons is quite random, we cna recognize them since they have duplicated end sequences in the place where they inserted

But consider that transposition is an evolutionary-sclae phenomenon: these object mutate very rapidly since no pressure and in many location we may find only remnants

thank you Daniela for re-post my question, I couldn't find the message anymore

thank you Professor, I got it

You're welcome :)

Gene activation by transposition of regulatory sequences has been described

also fusion of LINES with potentially oncogenic genes, see for example: doi: 10.1002/ijc.31831

an other question, the translation of transposase is physiologic in all organisms?

it happens very very rarely

can it be consider a mechanism for variability?

or only an error?

yes I think it concerns all organisms and it is for sure one of the mechnaisms of variability. If this is error or evolution is a philosophical questions, not my competence....

I think I'm not sure about how does a repetitive sequence look like

I mean, can a SINE or a LINE make a gene itself?

ok, I finish my question (for the moment), thank you!

LINEs contain genes, SINEs do not, please see slides of lesson 1

Okay thank you

I've got many other questions ahahah

OK if no other question I leave... sorry I have other duties now.

First, about the LAD's article:

What does it mean that “LADs make multivalent interactions with NL”?

Dear Daniela Penna, can you please put in the Forum ?

Yes, of course

sorry I must leave now

Thank you