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Friday, 6 March 2020, 11:35 AM --> Friday, 6 March 2020, 12:15 PM

11:35 Cecilia:

Anyway, if the professor will read our questions later mine are: 1) When transposons are translated they can recognize only the TIRs of their membership sequences or they can also relocate other sequences that contain TIRs 2) If only the promoter is transponed (?, is the correct terminology?), is it possible to have the unproperly translation of oncogene (tumor development)? So, we can consider trasposome like a tumor cause?

11:37: Allan Akandwanaho has just entered this chat
11:38 Allan:

good morning everyone

11:38 Cecilia:

Hi Allan

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11:39: Cecilia Boretto has just entered this chat
11:39 Allan:

i would like to ask if what exactly anotation means in genomes

11:42 Cecilia:

I'm not understanding

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11:45 Michele:

tell me what you need now

11:46 Cecilia:

Okay there are several question

11:46 Michele:

yes I've seen past questions

11:47: Daniela Penna has just entered this chat
11:47 Cecilia:

Great

11:47 Daniela:

Goddmorning prof

11:48 Allan:

i was wondering about anotation because the dictionary meaning is adding a comment to something

11:48 Allan:

if one says that genes are anotated, does it mean that they are commented

11:49 Allan:

sorry for the redundant question

11:49 Cecilia:

beyond those questions we were wondering why Activity 2 was reopened, do we have to do it again?

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11:51 Daniela:

I still can see my previous attempt

11:51 Michele:

i am engaged in a phone call at the moment, could you wait 5 min please

11:51 Michele:

no was for people that could'nt access before

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11:59 Michele:

OK i'm back

11:59 Michele:

concernign annotation: of course when scientists have a sequence of a fragment of DNA thay try to understand what it is

12:00 Michele:

e.g thay find similarity with a retrovirus, they call it "LTR"

12:00 Michele:

this is annotation

12:01 Michele:

unfortunately Allan has left

12:01 Michele:

OK any other question?

12:02 Daniela:

I'll copy and paste Cecilia's questions if you want

12:02 Daniela:

) When transposons are translated they can recognize only the TIRs of their membership sequences or they can also relocate other sequences that contain TIRs 2) If only the promoter is transponed (?, is the correct terminology?), is it possible to have the unproperly translation of oncogene (tumor development)? So, we can consider trasposome like a tumor cause?

12:02 Michele:

transposed

12:03 Michele:

Ye your guess is correct: the jumping of DNA transposons is quite random, we cna recognize them since they have duplicated end sequences in the place where they inserted

12:04 Michele:

But consider that transposition is an evolutionary-sclae phenomenon: these object mutate very rapidly since no pressure and in many location we may find only remnants

12:04 Cecilia:

thank you Daniela for re-post my question, I couldn't find the message anymore

12:05 Cecilia:

thank you Professor, I got it

12:05 Daniela:

You're welcome :)

12:05 Michele:

Gene activation by transposition of regulatory sequences has been described

12:06 Michele:

also fusion of LINES with potentially oncogenic genes, see for example: doi: 10.1002/ijc.31831

12:08 Cecilia:

an other question, the translation of transposase is physiologic in all organisms?

12:08 Michele:

it happens very very rarely

12:09 Cecilia:

can it be consider a mechanism for variability?

12:09 Cecilia:

or only an error?

12:10 Michele:

yes I think it concerns all organisms and it is for sure one of the mechnaisms of variability. If this is error or evolution is a philosophical questions, not my competence....

12:10 Daniela:

I think I'm not sure about how does a repetitive sequence look like

12:10 Daniela:

I mean, can a SINE or a LINE make a gene itself?

12:10 Cecilia:

ok, I finish my question (for the moment), thank you!

12:11 Michele:

LINEs contain genes, SINEs do not, please see slides of lesson 1

12:11 Daniela:

Okay thank you

12:12 Daniela:

I've got many other questions ahahah

12:12 Michele:

OK if no other question I leave... sorry I have other duties now.

12:12 Daniela:

First, about the LAD's article:

12:12 Daniela:

What does it mean that “LADs make multivalent interactions with NL”?

12:12 Michele:

Dear Daniela Penna, can you please put in the Forum ?

12:12 Daniela:

Yes, of course

12:12 Michele:

sorry I must leave now

12:12 Daniela:

Thank you

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