Instant Notes - Key concepts


1. Development

1.2. Fate and Commitment

As development proceeds trough cell proliferation, migration and differentiation, the number of different cell types in the embryo increases. Progenitor cells are progressively committed towards specific cell types.

Cell fate describes the range of cell types a progenitor cell can give rise to during normal development. 

Cell potency describes the  repertoire of cell types a progenitor cell can give rise to in all possible environments (e.g. a cell can differentiate in a different type compared to its "normal fate" if it is experimentally grafted in an ectopic region of the embryo). In animal development, cell potency is progressively restricted (from totipotent to unipotent) until a cell becomes terminally differentiated. 

As cell potency becomes restricted following each decision in the developmental hierarchy, cells are said to be committed to a certain fate and eventually differentiate in a specific cell type. 

Although commitment is a continuous process, developmental biologists identify different stages in cell commitment as shown in the figure below. 

A cell is said to be uncommitted if it has not yet received instruction directing it along a particular developmental pathway.
A cell is considered to be specified when it is directed to follow a certain developmental pathway and does so when it is  in its natural environment or it is placed in a neutral environment. At this stage, the cell  transplanted to an ectopic place can be re-specified toward a different fate by the action of external cues present in the new environment (cell commitment at this stage is reversible).
A cell is considered to be determined if its fate is fixed and can no longer be changed, regardless of the external cues. Determination to follow a specific developmental pathway coincides with loss of competence to follow alternative pathways.
But, are specialized cells irreversibly committed to their differentiated fate? think about it and read about experiments by John B. Gurdon and Shinya Yamanaka