BACKGROUND: DNA microarrays

Site: Cell Molecular Biology
Course: Advanced Molecular Biology 2019-2020
Book: BACKGROUND: DNA microarrays
Printed by: Guest user
Date: Wednesday, 24 April 2024, 5:47 PM

Description

Background knowledge on DNA microarrays

1. General microarray features

In general, the term DNA microarray refers to a small surface (glass slide or similar) to which a high number of single-stranded DNA molecules, usually not very long (25-200 bp depending on manufacturing process) are orderly attached (in the sense that we know each sequence and its position in the array. 

Probes can be derived from exons, or from any kind of other special interest sequences, suche as gene promoters, or enhancers and so on. Or even be derived from the entire genome sequence, without any bias, and with fixed spacing: for example, microarrays exist that contain probes for sequences of ca. 30 bp spaced regularly at 50bp intervals in the Human Genome. We call them "tiling microarrays". 

Special microarrays also can be made: for example, to study methylation, we can make probes that carry all possible C-->T conversion due to bisulfite treatment-PCR.   

I explored many web pages on microarrays, but at the end I think the most complete is still the Wikipedia page at: 

https://en.wikipedia.org/wiki/DNA_microarray

VIDEO 1: double-color

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VIDEO 2: single-color

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VIDEO 3 : details

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