BACKGROUND: NGS sequencing technologies

Site: Cell Molecular Biology
Course: Advanced Molecular Biology 2019-2020
Book: BACKGROUND: NGS sequencing technologies
Printed by: Guest user
Date: Wednesday, 24 April 2024, 6:22 PM

Description

Alcuni video e animazioni illustrano le principali tecnologie NGS

1. NGS Sequencing Technologies

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2. Surface technologies

Illumina technology requires in situ PCR in order to create DNA-nanoclones on a glass surface. It is illustrated in the following video: 

<iframe width="560" height="315" src="https://www.youtube.com/embed/womKfikWlxM" frameborder="0" allowfullscreen></iframe>




Helicos technology allows sequencing of single molecules with no PCR amplification step. See this  link:

<iframe width="560" height="315" src="https://www.youtube.com/embed/TboL7wODBj4" frameborder="0" allowfullscreen></iframe>


3. bead technologies

Roche 454 technology, which uses emulsion PCR amplification on beads followed by  Pyrosequencing  (see below) is available on this site: 

http://454.com/products/technology.asp

The following video is interesting but there is no explanation in the audio track  

<iframe width="560" height="315" src="https://www.youtube.com/embed/KzdWZ5ryBlA" frameborder="0" allow="autoplay; encrypted-media" allowfullscreen></iframe>


Pyrosequencing video :

4. Other technologies

Ion Torrent, is based on a H+ sensor (H+ is released at each poymerization step) does not require fluorochromes and optical systems. .

Emulsion PCR is on microbeads is required.

<iframe width="560" height="315" src="https://www.youtube.com/embed/DyijNS0LWBY" frameborder="0" allow="accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe>


5. Nanopore sequencing

Absolute novelty in the field id Oxford Nanopore Sequencing. Please follow this link:

https://nanoporetech.com/applications/dna-nanopore-sequencing