BACKGROUND: NGS sequencing technologies
Site: | Cell Molecular Biology |
Course: | Advanced Molecular Biology 2019-2020 |
Book: | BACKGROUND: NGS sequencing technologies |
Printed by: | Guest user |
Date: | Friday, 13 December 2024, 11:29 PM |
Description
Alcuni video e animazioni illustrano le principali tecnologie NGS
1. NGS Sequencing Technologies
<iframe width="560" height="315" src="https://www.youtube.com/embed/mI0Fo9kaWqo" frameborder="0" allow="accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe>
2. Surface technologies
Illumina technology requires in situ PCR in order to create DNA-nanoclones on a glass surface. It is illustrated in the following video:
<iframe width="560" height="315" src="https://www.youtube.com/embed/womKfikWlxM" frameborder="0" allowfullscreen></iframe>
Helicos technology allows sequencing of single molecules with no PCR amplification step. See this link:
<iframe width="560" height="315" src="https://www.youtube.com/embed/TboL7wODBj4" frameborder="0" allowfullscreen></iframe>
3. bead technologies
Roche 454 technology, which uses emulsion PCR amplification on beads followed by Pyrosequencing (see below) is available on this site:
http://454.com/products/technology.asp
The following video is interesting but there is no explanation in the audio track
<iframe width="560" height="315" src="https://www.youtube.com/embed/KzdWZ5ryBlA" frameborder="0" allow="autoplay; encrypted-media" allowfullscreen></iframe>
Pyrosequencing video :
4. Other technologies
Ion Torrent, is based on a H+ sensor (H+ is released at each poymerization step) does not require fluorochromes and optical systems. .
Emulsion PCR is on microbeads is required.
<iframe width="560" height="315" src="https://www.youtube.com/embed/DyijNS0LWBY" frameborder="0" allow="accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture" allowfullscreen></iframe>
5. Nanopore sequencing
Absolute novelty in the field id Oxford Nanopore Sequencing. Please follow this link:
https://nanoporetech.com/applications/dna-nanopore-sequencing