BACKGROUND: NGS sequencing technologies

1. NGS Sequencing Technologies

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2. Surface technologies

Illumina technology requires in situ PCR in order to create DNA-nanoclones on a glass surface. It is illustrated in the following video: 

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Helicos technology allows sequencing of single molecules with no PCR amplification step. See this  link:

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3. bead technologies

Roche 454 technology, which uses emulsion PCR amplification on beads followed by  Pyrosequencing  (see below) is available on this site: 

http://454.com/products/technology.asp

The following video is interesting but there is no explanation in the audio track  

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Pyrosequencing video :

4. Other technologies

Ion Torrent, is based on a H+ sensor (H+ is released at each poymerization step) does not require fluorochromes and optical systems. .

Emulsion PCR is on microbeads is required.

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5. Nanopore sequencing

Absolute novelty in the field id Oxford Nanopore Sequencing. Please follow this link:

https://nanoporetech.com/applications/dna-nanopore-sequencing