BACKGROUND: NGS sequencing technologies

1. Surface technologies

Illumina technology requires in situ PCR in order to create DNA-nanoclones on a glass surface. It is illustrated in the following video: 

<iframe width="560" height="315" src="https://www.youtube.com/embed/womKfikWlxM" frameborder="0" allowfullscreen></iframe>

Helicos technology allows sequencing of single molecules with no PCR amplification step. See this  link:

<iframe width="560" height="315" src="https://www.youtube.com/embed/TboL7wODBj4" frameborder="0" allowfullscreen></iframe>