Antibody production for experimental use
Immunohistochemistry
Immunohistochemistry
In the immunocytochemistry visual protocol video, you will learn how to clean and coat coverslips, prepare cell culture plates, and plate cells for ICC.In this video, you will learn how to coat coverships, prepare cell culture plats, plate cells for ICC, fix, permeabilize, block and add a primary antibody to your plated cells, add a secondary antibody, wash the plated cells, double label and mount your coverslips for analysis.
In this video you will learn how to add your secondary antibody, wash the plated cells, double label, and mount your coverslips onto slides for analysis.